CD137 Antibody

• Description:

  CD137 (4-1BB), a member of the tumor necrosis factor receptor superfamily, is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes. The functions of CD137 in T lymphocytes include regulating activation, proliferation and apoptosis. CD137 and CD28 are costimulatory molecules of T cell activation. Costimulatory molecules are important in initiating anti-tumor immune responses. CD137 plays an important role in regulating T-cell-dependent immune responses. Expression of CD137 correlates negatively with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and prolongs survival. The LOB12.3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling and delay tumor growthin vivowhen administered in combination with immune checkpoint inhibitors.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

• UniProt:

  P20334

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  A murine CD137 human Fc fusion protein was used as the immunogen for the CD137 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  LOB12

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD137 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD137 antibody is available for research use only.

• Storage Conditions:

  Store the CD137 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD137 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CD137 antibody (clone LOB12) as confirmation of integrity and purity.