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CD27 Antibody

CAT: 0800-V8229-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V8229-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P26842
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human CD27 protein was used as the immunogen for this CD27 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LPFS2/1611
Applications
ELISA, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD27 antibody is available for research use only.
Storage Conditions
Store the CD27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD27 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of human Ramos cells with CD27 antibody (clone LPFS2/1611, green) and Reddot nuclear stain (red) .

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