CD20 Antibody

• Description :

  Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12) . B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

• Specifications :

  Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood

• UniProt :

  P11836

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  B9E9

• Applications :

  FACS

• Purity :

  Protein G affinity chromatography

• Format :

  CF488 Conjugate

• Buffer :

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Store the CD20 antibody at 2-8oC, protected from light.

• Limitations :

  This CD20 antibody is available for research use only.

• Storage Conditions :

  Store the CD20 antibody at 2-8°C, protected from light.

• Formulation :

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD20 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic