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GCLM Antibody

CAT: 0800-V8219-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V8219-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P48507
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CPTC-GCLM-1
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the GCLM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This GCLM antibody is available for research use only.
Storage Conditions
Store the GCLM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the GCLM antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Product Datasheet
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