GCLC Antibody

• Description :

  Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow Cytometry: 1:10-1:50

• UniProt :

  P48506

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 1-30 from the human protein was used as the immunogen for this GCLC antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, IF, FACS, ELISA

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the GCLC antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This GCLC antibody is available for research use only.

• Storage Conditions :

  Aliquot the GCLC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the GCLC antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Confocal immunofluorescent analysis of GCLC antibody with U-251MG cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) . DAPI was used as a nuclear counterstain (blue) .