VIM Antibody / Vimentin

• Description:

  The VIM gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. [RefSeq]

• Specifications:

  Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50-1:200

• UniProt:

  P08670

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A synthetic peptide specific to human Vimentin / VIM was used as the immunogen for the VIM antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  AGF-22

• Type:

  Recombinant

• Applications:

  IHC-P, WB, IF

• Purity:

  Affinity purified

• Format:

  Purified

• Buffer:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA

• Reconstitution:

  Store the VIM antibody at -20oC.

• Limitations:

  This VIM antibody is available for research use only.

• Storage Conditions:

  Store the VIM antibody at -20°C.

• Formulation:

  Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA

• Applications Notes:

  Optimal dilution of the VIM antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic, nuclear, cell membrane

• Image Legend:

  IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.