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VIM Antibody / Vimentin

CAT: 0800-RQ5250Size: 100 µLDry Ice: NoHazardous: No
CAT#:0800-RQ5250Size:100 µL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The VIM gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. [RefSeq]
CAS Number
9000-83-3
Specifications
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:200, Immunofluorescence: 1:50-1:200
UniProt
P08670
Host
Rabbit
Reactivity
Human
Immunogen
A synthetic peptide specific to human Vimentin / VIM was used as the immunogen for the VIM antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
AGF-22
Type
Recombinant
Applications
IHC-P, WB, IF
Purity
Affinity purified
Format
Purified
Buffer
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA
Reconstitution
Store the VIM antibody at -20oC.
Limitations
This VIM antibody is available for research use only.
Storage Conditions
Store the VIM antibody at -20°C.
Formulation
Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5 mg/mL BSA
Applications Notes
Optimal dilution of the VIM antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear, cell membrane
Image Legend
IHC staining of FFPE human colon tissue with VIM antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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