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Granzyme B Antibody

CAT: 0800-V8027BTNSize: 500 µLDry Ice: NoHazardous: No
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CAT#:0800-V8027BTNSize:500 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface s Disease.
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT
UniProt
P10144
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 73-187 from the human protein was used as the immunogen for the Granzyme B antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
GZMB/3014
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Biotin Conjugate
Buffer
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Granzyme B antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
Limitations
This Granzyme B antibody is available for research use only.
Storage Conditions
Store the Granzyme B antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .
Formulation
0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Granzyme B antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human spleen with biotinylated Granzyme B antibody (clone GZMB/3014) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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