PD-L1 Antibody / B7-H1 / CD274

• Description:

  Engagement of CD28 by B7-1 (CD80) or B7-2 (CD86) in the presence of antigen promotes T-cell proliferation, cytokine production, differentiation of effector T-cells and the induction of BCLX, a promoter of T-cell survival. Engagement of CTLA4 by B7-1 or B7-2, on the other hand, may inhibit proliferation and interleukin-2 (IL-2) production. PD-L1 is 290-amino acid type I transmembrane protein, which is 20% and 15% identical to B7-1 and B7-2, respectively, has immunoglobulin V-like and C-like domains and a 30-amino acid cytoplasmic tail. PD-L1 does not bind CD28, cytotoxic T-lymphocyte A4 or ICOS (inducible co-stimulator) . IL-2, although produced in small amounts, is required for the effect of PD-L1 co-stimulation. PD-L2 protein contains a signal sequence, IgV- and IgC-like domains, a transmembrane region and a cytoplasmic region. The constitutive expression of PD-L1 and PD-L2 on parenchymal cells of heart, lung and kidney suggests that the PD-1-PD-L system could provide unique negative signaling to help prevent autoimmune diseases.

• Specifications:

  ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL

• UniProt:

  Q9NZQ7

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  A recombinant human partial protein (amino acids 39-191) was used as the immunogen for the PD-L1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  PDL1/2744

• Applications:

  ELISA, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the PD-L1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PD-L1 antibody is available for research use only.

• Storage Conditions:

  Store the PD-L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the PD-L1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Western blot testing of human and mouse samples with PD-L1 antibody (clone PDL1/2744) . Predicted molecular weight ~34 kDa (unmodified), 45-70 kDa (glycosylated) .