HER2 Antibody / ErbB2

• Description :

  Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  P04626

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant human HER2 protein fragment within amino acids 311-462 was used as the immunogen for the HER2 antibody. Its epitope is localized in the extracellular domain.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  ERBB2/3079

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the HER2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This HER2 antibody is available for research use only.

• Storage Conditions :

  Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the HER2 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cell surface

• Image Legend :

  IHC staining of FFPE human breast carcinoma with HER2 antibody (clone ERBB2/3079) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.