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HER2 ErbB2 Antibody

CAT: 0800-V2111-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2111-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. HER2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 / ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
CAS Number
9007-83-4
Specifications
ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 0.5-1 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human HER2 protein was used as the immunogen for this antibody. (1) Its epitope is localized in the extracellular domain.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
HRB2/718
Applications
ELISA, FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HER2 ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HER2 / ErbB2 antibody is available for research use only.
Storage Conditions
Store the HER2 ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. This HER2 antibody binds to the extracellular/cell surface region of the protein.
Location
Extracellular/Intracellular cell membrane
Image Legend
Flow cytometry testing of human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/718) ; Red=isotype control, Blue= HER2 ErbB2 antibody.
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