HER2 ErbB2 Antibody

• Description:

  This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. HER2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 / ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

• Specifications:

  ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 0.5-1 µg/mL

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human HER2 protein was used as the immunogen for this antibody. (1) Its epitope is localized in the extracellular domain.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  HRB2/718

• Applications:

  ELISA, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the HER2 ErbB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HER2 / ErbB2 antibody is available for research use only.

• Storage Conditions:

  Store the HER2 ErbB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. This HER2 antibody binds to the extracellular/cell surface region of the protein.

• CAS Number:

  9007-83-4

• Location:

  Extracellular/Intracellular cell membrane

• Image Legend:

  Flow cytometry testing of human MCF7 cells with HER2 ErbB2 antibody (clone HRB2/718) ; Red=isotype control, Blue= HER2 ErbB2 antibody.