ATG5 Antibody

CAT: 0800-V7623-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V7623-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. The ATG5 protein is essential for autophagy; a process that is usually beneficial for cells to self-degrade their own components when they are no longer useful.
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q9H1Y0
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 1-119) was used as the immunogen for the ATG5 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ATG5/2492
Applications
ELISA, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the ATG5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This ATG5 antibody is available for research use only.
Storage Conditions
Store the ATG5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the ATG5 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human colon carcinoma with ATG5 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
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