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ATG7 Antibody

CAT: 0800-RQ5947Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ5947Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O95352
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids HELTQKKLNEYRLDEAPKDIK from the human protein were used as the immunogen for the ATG7 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the ATG7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ATG7 antibody is available for research use only.
Storage Conditions
After reconstitution, the ATG7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ATG7 antibody should be determined by the researcher.
Image Legend
Flow cytometry testing of human HeLa cells with ATG7 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= ATG7 antibody.
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