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Recombinant Golgi Antibody

CAT: 0800-V7503-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7503-100UGSize:100 µg
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Description
This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q92896
Host
Rabbit
Reactivity
Human
Immunogen
Full length recombinant GLG1 protein was used as the immunogen for this recombinant Golgi antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
GLG1/2829R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Golgi antibody is available for research use only.
Storage Conditions
Store the recombinant Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Golgi complex in cytoplasm
Image Legend
IHC staining of FFPE human breast carcinoma with recombinant Golgi antibody (clone GLG1/2829R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

UniProtKB · Q92896

Golgi apparatus protein 1

GSLG1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q92896
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GLG1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B7Z8Y4, D3DUJ7, Q13221, Q6P9D1
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmCytoskeletonGolgi apparatusMembrane

PTM

GlycoproteinPhosphoprotein

Technical term

Proteomics identificationReference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

Ligand

Sialic acid

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