Golgi Antibody

• Description:

  This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunocytochemistry (Acetone or paraformaldehyde fixed) : 1-2 µg/mL for 30 min, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Not Known

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  AE-6

• Applications:

  FACS, IF, WB, ICC, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Golgi antibody is available for research use only.

• Storage Conditions:

  Store the Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Golgi complex in cytoplasm

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human pancreas stained with Golgi antibody (AE-6) .