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Recombinant CD44 Antibody

CAT: 0800-V7500IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V7500IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44/HCAM immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.
CAS Number
9000-83-3
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P16070
Host
Rabbit
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the recombinant CD44 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
HCAM/2875R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the recombinant CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD44 antibody is available for research use only.
Storage Conditions
Store the recombinant CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cell surface, cytoplasmic
Image Legend
IHC staining of FFPE human lymph node with recombinant CD44 antibody (clone HCAM/2875R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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