Recombinant CD44 Antibody

• Description :

  Recognizes a cell surface glycoprotein of 80-95kDa (CD44/HCAM) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44/HCAM immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

• CAS Number :

  9000-83-3

• Specifications :

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt :

  P16070

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Stimulated human leukocytes were used as the immunogen for the recombinant CD44 antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG κ

• Clone :

  HCAM/2875R

• Type :

  Recombinant

• Applications :

  IHC-P

• Purity :

  Protein A affinity chromatography

• Format :

  Purified

• Buffer :

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution :

  Store the recombinant CD44 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This recombinant CD44 antibody is available for research use only.

• Storage Conditions :

  Store the recombinant CD44 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC staining of FFPE human lymph node with recombinant CD44 antibody (clone HCAM/2875R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.