Recombinant TOP1MT Antibody

• Description:

  DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  Q969P6

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the recombinant TOP1MT antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  TPIMT-2R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the recombinant TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant TOP1MT antibody is available for research use only.

• Storage Conditions:

  Store the recombinant TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic (mitochondria)

• Image Legend:

  IHC staining of FFPE human pancreas with recombinant TOP1MT antibody (clone TPIMT-2R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.