TOP1MT Antibody

• Description :

  DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt :

  Q969P6

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human TOP1MT protein was used as the immunogen for the TOP1MT antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  TOP1MT/613

• Applications :

  WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This TOP1MT antibody is available for research use only.

• Storage Conditions :

  Store the TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the TOP1MT antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic (Mitochondria)

• Image Legend :

  IHC staining of FFPE human melanoma with TOP1MT antibody (clone TOP1MT/613) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.