TOP1MT Antibody

• Description :

  DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

• Specifications :

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt :

  Q969P6

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  TOP1MT/488

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution :

  Store the TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This TOP1MT antibody is available for research use only.

• Storage Conditions :

  Store the TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic (mitochondria)

• Image Legend :

  IHC: Formalin-fixed, paraffin-embedded human melanoma stained with TOP1MT antibody (clone TOP1MT/488) .