Recombinant CD34 Antibody

CAT: 0800-V7384SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V7384SAF-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
CAS Number
9000-83-3
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P28906
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
HPCA1/2598R
Type
Recombinant
Applications
FACS, IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD34 antibody is available for research use only.
Storage Conditions
Store the recombinant CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cell surface, cytoplasmic
Image Legend
IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

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