CD34 Antibody

CAT: 0800-V4789-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V4789-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. It appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P28906
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the CD34 antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
CD34/7721
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD34 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD34 antibody is available for research use only.
Storage Conditions
Aliquot the CD34 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD34 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human placental tissue with CD34 antibody (clone CD34/7721) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Product Datasheet
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