Recombinant CD34 Antibody

• Description :

  This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.

• Specifications :

  Flow cytometry: 0.5-1ug/10^6 cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P28906

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG κ

• Clone :

  HPCA1/2598R

• Type :

  Recombinant

• Applications :

  FACS, IHC-P

• Purity :

  Protein A affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the recombinant CD34 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This recombinant CD34 antibody is available for research use only.

• Storage Conditions :

  Store the recombinant CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9000-83-3

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC testing of FFPE human renal cell carcinoma with recombinant CD34 antibody (clone HPCA1/2598R) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.