Recombinant Bcl6 Antibody

• Description:

  Bcl6 is a transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. [UniProt]

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P41182

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 256-389 were used as the immunogen for the recombinant Bcl6 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ARBC6-2R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the recombinant Bcl6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant Bcl6 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Bcl6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9000-83-3

• Location:

  Nuclear

• Image Legend:

  IHC testing of FFPE human tonsil with recombinant Bcl6 antibody (clone ARBC6-2R) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.