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Recombinant IgM Antibody

CAT: 0800-V7292IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V7292IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
CAS Number
9000-83-3
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P01871, P20769
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant heavy chain of human IgM was used as the immunogen for this recombinant IgM antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
IGHM/2559R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the recombinant IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant IgM antibody is available for research use only.
Storage Conditions
Store the recombinant IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasm, cell surface, secreted
Image Legend
IHC staining of FFPE human tonsil tissue with recombinant IgM antibody (clone IGHM/2559R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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