Elastase 3B Antibody

• Description:

  Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P08861

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ELTS3B-2

• Applications:

  IHC-P

• Purity:

  Protein G

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Elastase 3B antibody is available for research use only.

• Storage Conditions:

  Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Elastase 3B antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone ELTS3B-2) . FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.