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Elastase 3B Antibody

CAT: 0800-V7213-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V7213-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P08861
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
ELTS3B-2
Applications
IHC-P
Purity
Protein G
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Elastase 3B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Elastase 3B antibody is available for research use only.
Storage Conditions
Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Elastase 3B antibody should be determined by the researcher.
Image Legend
IHC testing of FFPE rat pancreas with Elastase 3B antibody (clone ELTS3B-2) . FFPE staining requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

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