CD74 Antibody

• Description:

  CD74 associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta) . Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [RefSeq]

• Specifications:

  Flow Cytometry: 0.5-1ug/million cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry: 1-2 µg/mL, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt:

  P04233

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human protein was used as the immunogen for the CD74 antibody. The epitope is known to be extracellular.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CDLA74-1

• Applications:

  FACS, IF, IHC

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD74 antibody is available for research use only.

• Storage Conditions:

  Store the CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface and paranuclear globular

• Image Legend:

  Flow cytometry testing of human Raji cells with CD74 antibody (clone CDLA74-1) ; Red=isotype control, Blue= CD74 antibody.