VEGF Antibody / VEGFA

• Description:

  Vascular Endothelial Growth Factor is a member of the PDGF/VEGF growth factor family. It is a heparin-binding protein which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. [RefSeq]

• Specifications:

  Immunohistochemistry (FFPE) : 4-8 µg/mL for 30 min at RT (1)

• UniProt:

  P15692

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human recombinant protein was used as the immunogen for the VEGF antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  VGFA-1

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This VEGF antibody is available for research use only.

• Storage Conditions:

  Store the VEGF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Titering of the VEGF antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, cell surface and extracellular (secreted)

• Image Legend:

  IHC testing of FFPE human ovarian carcinoma with VEGF antibody (clone VGFA-1) .