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VEGF Antibody / VEGFA

CAT: 0800-V7175-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7175-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Vascular Endothelial Growth Factor is a member of the PDGF/VEGF growth factor family. It is a heparin-binding protein which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. [RefSeq]
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 4-8 µg/mL for 30 min at RT (1)
UniProt
P15692
Host
Mouse
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the VEGF antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VGFA-1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This VEGF antibody is available for research use only.
Storage Conditions
Store the VEGF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the VEGF antibody may be required for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 8, for 10-20 min followed by cooling at RT for 20 min.
Location
Cytoplasmic, cell surface and extracellular (secreted)
Image Legend
IHC testing of FFPE human ovarian carcinoma with VEGF antibody (clone VGFA-1) .

UniProtKB · P15692

Vascular endothelial growth factor A, long form

VEGFA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P15692
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VEGFA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
L-VEGF
EC number
—
Processing
Precursor
Secondary accessions
B5BU86, H0Y2S8, H0Y407, H0Y414, H0Y462, H0Y8N2, H3BLW7, O60720, O75875, Q074Z4, Q16889, Q5UB46, Q6P0P5, Q96KJ0, Q96L82, Q96NW5, Q9H1W8, Q9H1W9, Q9UH58, Q9UL23
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative initiationAlternative promoter usageAlternative splicing

Biological process

AngiogenesisDifferentiation

Cellular component

CytoplasmEndoplasmic reticulumExtracellular matrixGolgi apparatusNucleusSecreted

Molecular function

Developmental proteinGrowth factorHeparin-bindingMitogen

PTM

Disulfide bondGlycoprotein
Product Datasheet
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