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VEGF Antibody

CAT: 0800-V8154SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8154SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF) . It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF) . There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 2-4 µg/mL
UniProt
P15692
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the VEGF antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VG1
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This VEGF antibody is available for research use only.
Storage Conditions
Store the VEGF antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the VEGF antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human tonsil with VEGF antibody (clone VG1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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