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HSP27 Antibody

CAT: 0800-V7102-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V7102-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HSP27 is induced by environmental stress and developmental changes. It is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. [RefSeq]
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P04792
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human HSPB1 protein was used as the immunogen for the HSP27 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SRPR27
Applications
IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the HSP27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HSP27 antibody is available for research use only.
Storage Conditions
Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Predominantly cytoplasmic with some nuclear
Image Legend
IHC testing of FFPE human prostate carcinoma and HSP27 antibody (clone SRPR27) . Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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