SUMO-2 Antibody

CAT: 0800-V7051-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V7051-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P61956
Host
Mouse
Reactivity
Human, Rat
Immunogen
Recombinant human protein was used as the immunogen for the SUMO-2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
S2MT-1
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the SUMO-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This SUMO-2 antibody is available for research use only.
Storage Conditions
Store the SUMO-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the SUMO-2 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Nuclear
Image Legend
IHC testing of formalin-fixed, paraffin-embedded human tonsil with SUMO-2 antibody (clone S2MT-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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