CD79a Antibody

• Description:

  A disulphide-linked heterodimer consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, CD79 is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with RituximAb (anti-CD20) . This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.

• Specifications:

  Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

• UniProt:

  P11912

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A soluble form of the extracellular IgSF domain from the human protein was used as the immunogen for the CD79a antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CDLA79a-1

• Applications:

  IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD79a antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD79a antibody is available for research use only.

• Storage Conditions:

  Store the CD79a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Immunofluorescent staining of PFA-fixed human Raji cells with CD79a antibody (green, clone CDLA79a-1) and Reddot nuclear stain (red) .