CD79b Antibody

• Description:

  Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. [UniProt]

• Specifications:

  IHC (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P40259

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 29-159 was used as the immunogen for the CD79b antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CDLA79b-1

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD79b antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD79b antibody is available for research use only.

• Storage Conditions:

  Store the CD79b antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the CD79b antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC testing of FFPE human tonsil tissue with CD79b antibody (clone CDLA79b-1) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.