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Perforin Antibody / PRF1

CAT: 0800-V3907SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3907SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9) . Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P14222
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 413-552 from the human protein was used as the immunogen for the Perforin antibody.
Clonality
Monoclonal
Isotype
IgG2c κ
Clone
PRF1/2470
Applications
IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Perforin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Perforin antibody is available for research use only.
Storage Conditions
Store the Perforin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Perforin antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human spleen with Perforin antibody (clone PRF1/2470) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · P14222

Perforin-1

PERF_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14222
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRF1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
P1
EC number
—
Processing
Precursor
Secondary accessions
B2R6X4, Q59F57, Q86WX7
Protein keywords

Ligand

CalciumMetal-binding

Cellular component

Cell membraneEndosomeLysosomeMembraneSecreted

Biological process

Cytolysis

Disease

Disease variantFamilial hemophagocytic lymphohistiocytosis

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignalTransmembraneTransmembrane beta strand

Technical term

Proteomics identificationReference proteome

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