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Perforin Antibody

CAT: 0800-R31574Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31574Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Perforin-1 is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, it inserts itself into the target cells plasma membrane, forming a pore. The lytic membrane-inserting part of Perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9) . Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
Host
Rabbit
Reactivity
Human
Immunogen
Human partial recombinant protein (AA 175-555) was used as the immunogen for this Perforin antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the Perforin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Perforin antibody is available for research use only.
Storage Conditions
After reconstitution, the Perforin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the Perforin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of Perforin antibody and Lane 1: HeLa; 2: COLO320; 3: HEPG2. Predicted/observed size ~61KD
Product Datasheet
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