OLIG2 Antibody

• Description:

  Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis) .

• Specifications:

  IHC (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Q13516

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 1-141 from the human protein was used as the immunogen for this OLIG2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ODTF2-1

• Applications:

  IHC-P

• Purity:

  Protein G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  After reconstitution, the OLIG2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This OLIG2 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the OLIG2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the OLIG2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC testing of FFPE human cerebellum with OLIG2 antibody (clone ODTF2-1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.