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CD3 epsilon Antibody

CAT: 0800-V3761SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3761SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes the epsilon chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 23-119 were used as the immunogen for the CD3 epsilon antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CDLA3e-1
Applications
FACS, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD3 epsilon antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD3 epsilon antibody is available for research use only.
Storage Conditions
Store the CD3 epsilon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The stated application concentrations are suggested starting points. Titration of the CD3 epsilon antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cell surface and cytoplasmic
Image Legend
Western blot testing of human Jurkat cells with CD3e antibody (clone CDLA3e-1) . Expected molecular weight ~23 kDa.

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