Products for Research Use Only
RegisterLogin

Recombinant CD20 Antibody

CAT: 0800-V3727-100UGSize: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V3727-100UGSize:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This mAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
CAS Number
9000-83-3
Specifications
Immunofluorescence: 1-3 µg/mL, Flow cytometry: 1-2ug/million cells, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P11836
Host
Mouse
Reactivity
Human
Immunogen
Human CD20 protein was used as the immunogen for this recombinant CD20 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RIGEL/773
Type
Recombinant
Applications
IF, FACS, IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Recombinant CD20 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This recombinant CD20 antibody is available for research use only.
Storage Conditions
Recombinant CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the recombinant CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Predominantly cell surface with some cytoplasmic
Image Legend
IHC testing of FFPE human tonsil tissue with recombinant CD20 antibody (clone rIGEL/773) . Required HIER: steam section in pH6 citrate buffer for 20 min.

Related Products

CatalogName
R33308-100UGCD20 Antibody
V9456SAF-100UGCD20 Antibody
V4166-20UGCD20 Antibody
V2048CF488-100TCD20 Antibody
V4167-20UGCD20 Antibody
RQ5378CD20 Antibody
V3245-100UGCD20 Antibody
V7618IHC-7MLCD20 Antibody
V2398-20UGCD20 Antibody
V8375SAF-100UGCD20 Antibody

Alternative Products

CatalogName
RAC07099-01Cd20 Recombinant Antibody
GTR18573647Recombinant CD20 Antibody
PT_40130-01CD20 Recombinant monoclonal Anti-Human (CD20 antibody)
AN300908L-01Recombinant CD20 Monoclonal Antibody
RAA00251-01CD20 Recombinant Monoclonal Antibody
OAAV01279-200UGCD20 Recombinant Antibody [18B12]
AE00144CD20 Recombinant Antibody AE00144
IRS112MSCD20 Recombinant Chimeric Antibody Antibody
V0019-01CD20 [ABT-CD20] Antibody
PX-P3060-50CD20 Protein - Human CD20 Recombinant Protein

Popular Products