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NKX2.2 Antibody

CAT: 0800-V3726IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V3726IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
O95096
Host
Mouse
Reactivity
Human
Immunogen
A human partial recombinant protein corresponding to amino acids 1-119 was used as the immunogen for this NKX2.2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
RMNK2-2
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NKX2.2 antibody is available for research use only.
Storage Conditions
Store the NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC testing of FFPE Ewings sarcoma with NKX2.2 antibody (clone RMNK2-2) . HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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