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Recombinant Human IgG Antibody

CAT: 0800-V3583-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3583-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P01857
Host
Mouse
Reactivity
Human
Immunogen
Human Ig Gamma Chain was used as the immunogen for this recombinant human IgG antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RIG266
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant human IgG antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant human IgG antibody is available for research use only.
Storage Conditions
Store the recombinant human IgG antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the recombinant human IgG antibody for each application should be determined by the researcher.
Location
Cytoplasm, Cell Surface and Secreted
Image Legend
IHC testing of FFPE human tonsil with recombinant human IgG antibody (clone rIG266) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · P01857

Immunoglobulin heavy constant gamma 1

IGHG1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01857
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IGHG1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A0A0A0MS08
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Cellular component

Cell membraneImmunoglobulinMembraneSecreted

PTM

Disulfide bondGlycoprotein

Domain

Immunoglobulin domainTransmembraneTransmembrane helix
Product Datasheet
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