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S100B Antibody

CAT: 0800-V3506SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3506SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker. [Wiki]
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistology (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P04271
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human S100B protein was used as the immunogen for the S100B antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
PS1B1-1
Applications
IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the S100B antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This S100B antibody is available for research use only.
Storage Conditions
Store the S100B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the S100B antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cytoplasmic, nuclear
Image Legend
Confocal Immunofluorescent analysis of A2058 cells using Alexa Fluor 488-labeled S100B antibody (green) . F-actin filaments were labeled with DyLight 554 Phalloidin (red) . DAPI was used to stain the cell nuclei (blue) .

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