SOX2 Antibody

• Description:

  SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.

• Specifications:

  ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P48431

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Amino acids 176-305 of the human protein were used as the immunogen for the SOX2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  SOX2/1792

• Applications:

  ELISA, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the SOX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This SOX2 antibody is available for research use only.

• Storage Conditions:

  Store the SOX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the SOX2 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC testing of FFPE human cervical carcinoma with SOX2 antibody (clone SOX2/1792) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.