E. coli Antibody

• Description:

  This mAb shows specificity to E. coli in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC) . Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.

• Specifications:

  ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format), Immunofluorescence: 1-2 µg/mL

• Host:

  Mouse

• Reactivity:

  E. coli

• Immunogen:

  A crude sonicate of E. coli was used as the immunogen for the E. coli antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgM k

• Clone:

  9.88

• Applications:

  ELISA, IF

• Purity:

  PEG precipitation followed by dialysis in 1X PBS

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the E. coli O157 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This E coli O157 antibody is available for research use only.

• Storage Conditions:

  Store the E. coli antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the E. coli antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Whole organism