E. coli Antibody

CAT: 0800-V3307SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V3307SAF-100UGSize:100 µg
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Description
This mAb shows specificity to E. coli in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms) . Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56) . The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC) . Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
CAS Number
9007-83-4
Specifications
ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format), Immunofluorescence: 1-2 µg/mL
Host
Mouse
Reactivity
E. coli
Immunogen
A crude sonicate of E. coli was used as the immunogen for the E. coli antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
9.88
Applications
ELISA, IF
Purity
PEG precipitation followed by dialysis in 1X PBS
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the E. coli O157 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This E coli O157 antibody is available for research use only.
Storage Conditions
Store the E. coli antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the E. coli antibody should be determined by the researcher.
Location
Whole organism

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