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E Cadherin Antibody

CAT: 0800-F44156-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F44156-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, Flow Cytometry: 1:10-1:50
UniProt
P12830
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 160-189 from the human protein was used as the immunogen for this E Cadherin antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the E Cadherin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This E Cadherin antibody is available for research use only.
Storage Conditions
Aliquot the E Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the E Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Cytoplasmic, membranous
Image Legend
Western blot analysis of E Cadherin antibody and 293 lysate. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level) .

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