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Cyclophilin E Antibody

CAT: 0800-RQ6110Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6110Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate
UniProt
Q9UNP9
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A human recombinant partial protein (amino acids M1-V301) was used as the immunogen for the Cyclophilin E antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, IP, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Cyclophilin E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cyclophilin E antibody is available for research use only.
Storage Conditions
After reconstitution, the Cyclophilin E antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Cyclophilin E antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human A431 cells with Cyclophilin E antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.
Product Datasheet
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