Cyclophilin E Antibody

• Description :

  Peptidylprolyl isomerase E (cyclophilin E), also known as PPIE, is an enzyme which in humans is encoded by the PPIE gene on chromosome 1. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.

• CAS Number :

  9007-83-4

• Specifications :

  Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunoprecipitation: 2ug per 500ug of lysate

• UniProt :

  Q9UNP9

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  A human recombinant partial protein (amino acids M1-V301) was used as the immunogen for the Cyclophilin E antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB, IF, FACS, IP, Direct ELISA

• Purity :

  Affinity purified

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution :

  After reconstitution, the Cyclophilin E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This Cyclophilin E antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the Cyclophilin E antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  Optimal dilution of the Cyclophilin E antibody should be determined by the researcher.

• Location :

  Nuclear

• Image Legend :

  Immunofluorescent staining of FFPE human A431 cells with Cyclophilin E antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.