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E-Cadherin Antibody

CAT: 0800-F54046-0.1MLSize: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0800-F54046-0.1MLSize:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 1:1000
UniProt
P12830
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone
6B10-F4-G10
Applications
WB
Purity
Protein G affinity
Format
Purified
Buffer
Antibody in PBS with 50% Glycerol and 0.03% ProClin 300
Reconstitution
Store the E-Cadherin antibody at -20oC.
Limitations
This E-Cadherin antibody is available for research use only.
Storage Conditions
Store the E-Cadherin antibody at -20°C.
Formulation
In PBS with 50% glycerol and 0.03% ProClin 300
Applications Notes
The stated application concentrations are suggested starting points. Titration of the E-Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of human 1) T47D, 2) HCT116, 3) MDA-MB-468 and 4) Caco-2 cell lysates using E-Cadherin antibody at 1:2000. Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on glycosylation level) .

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