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Clathrin Light Chain Antibody

CAT: 0800-V3300-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V3300-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B) . Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09496, P09497
Host
Mouse
Reactivity
Human
Immunogen
A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM174
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Clathrin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Clathrin Light Chain antibody is available for research use only.
Storage Conditions
Store the Clathrin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone SPM174) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

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