Clathrin Heavy Chain Antibody

• Description:

  Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.

• Specifications:

  Immunofluorescence: 1-2 µg/mL

• UniProt:

  Q00610, P53675

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human Clathrin Heavy Chain protein was used as the immunogen for this Clathrin Heavy Chain antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CHC/1432

• Applications:

  IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the Clathrin Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Clathrin Heavy Chain antibody is available for research use only.

• Storage Conditions:

  Store the Clathrin Heavy Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Immunofluorescent staining of permeabilized human HeLa cells with Clathrin Heavy Chain antibody (clone CHC/1432, green) and Reddot nuclear stain (red) .