B-Catenin Antibody

• Description:

  The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs) . AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

• UniProt:

  P35222

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Chicken b-Catenin was used as the immunogen for the b-Catenin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  9F2

• Applications:

  FACS, IF, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the b-Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This b-Catenin antibody is available for research use only.

• Storage Conditions:

  Store the b-Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titration of the b-Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic, cell junctions

• Image Legend:

  Western blot testing of human 1) A431, 2) A549 and 3) MCF7 cell lysate with Beta Catenin antibody (clone 9F2) . Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.