Alpha Catenin Antibody

• Description :

  Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-Catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, alpha-Catenin forms a heterodimer with beta-Catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-Catenin also regulates the beta-Catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.

• CAS Number :

  9007-83-4

• Specifications :

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

• UniProt :

  P35221

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A human fusion protein was used as the immunogen for the Alpha Catenin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  1G5

• Applications :

  FACS, IF, WB

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the Alpha Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Alpha Catenin antibody is available for research use only.

• Storage Conditions :

  Store the Alpha Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the Alpha Catenin antibody to be determined by the researcher.

• Location :

  Cell surface, cytoplasmic, cell junctions

• Image Legend :

  Western blot testing of human 1) A431 and 2) MCF7 cell lysate with Alpha Catenin antibody (clone 1G5) . Expected molecular weight ~102 kDa.